The edgun regulator testers will work with all the matador, morana, and lelya regulators from the R2.5 though the R5M since Ed has used the same basic regulator and air reservoir diameter for quite a while now. The factory edgun regulators have always been quite reliable and consistent, and with their larger size they're very easy to work on since there are no tiny o rings to fight with.
You don't need a regulator tester to tune the rifle, but they certainly make it nice to check or compare regulators between different rifles like you want to do, and they save you a lot of time by allowing you to set the regulator to the exact previous setting after a regulator rebuild. Setting the piston to the same measured depth after a rebuild usually gets the regulator setting pretty close, but the tester lets you know for sure.
If you don't have a tester, you could swap regulators between rifles and see what happens, watching for the velocity change on the chronograph to see when the rifle falls off the regulator to roughly measure the regulator setting, or check the belleville stacks and piston depth between the two regulators as a rough comparison of set points... but a regulator tester would let you know for sure if the two regulators have wildly different set points.